The Rees Lab

Home

Research Interests

Group Members

Recent Publications

Courses

Related Links

Group Member Login

NOTE: Link to a pdf version of a publication using the DOI link or a free PMC article using the PUBMED link.

Lai, J.Y., Poon, Y.S., Kaiser, J T. and Rees, D.C. "Open and Shut: Crystal Structures of the Dodecylmaltoside Solubilized Mechanosensitive Channel of Small Conductanse from E. coli and H. pylori at 4.4 Å and 4.1 Å Resolution" Protein Science 22, 502-209 (2013). doi: 10.1002/pro.2222 PUBMED abstract

Johnson, E., Nguyen, P.T., Yeates, T.O., and Rees, D. C. “Inward Facing Conformations of the MetNI Methionine ABC Transporters: Implications for the Mechanism of Transinhibition.” Protein Science 21, 84-96 (2012). doi: 10.1002/pro.765 PUBMED

Bobyr, E., Lassila, J.K., Wiersma-Koch, H.I., Fenn, T.D., Lee, J.J., Nikolic-Hughes, I., Hodgson, K.O., Rees, D.C., Hedman, B. and Herschlag, D. "High-resolution Analysis of Zn2+ Coordination in the Alkaline Phosphatase Superfamily by EXAFS and X-ray Crystallography” J. Mol. Biol. 415, 102-117 (2012). doi: 10.1016/j.jmb.2011.10.040 PUBMED abstract

Tirado-Lee, L., Lee, A., Rees, D.C. and Pinkett, H.W. "Classification of a Haemophilus influenzae ABC Transporter HI1470/71 Through Its Cognate Molybdate Periplasmic Binding Protein, MolA" Structure 19, 1701-1710 (2011) doi: 10.1016/j.str.2011.10.004 PUBMED

Spatzal, T., Aksoyoglu, M., Zhang, L., Andrade, S.L.A., Schleicher, E., Weber, S., Rees, D.C. and Einsle, O. "Evidence for Interstitial Carbon in Nitrogenase FeMo Cofactor" Science 334, 940 (2011). doi: 10.1126/science.1214025 PUBMED

Haswell, E.S., Phillips, R. and Rees, D.C. "Mechanosensitive Channels: What Can They Do and How Do They Do It?" Structure 19, 1356-1369 (2011). doi: 10.1016.j.str.2011.09.005 PUBMED

Kaiser, J. T., Hu, T., Wiig. J. A., Rees, D. C., and Ribbe, M. W. "Structure of Precursor Bound NifEN: a Nitrogenase FeMo Cofactor maturase/insertase" Science 331, 91-94 (2011). doi:10.1126/science.1196954 PUBMED

Gandhi, C. S., Walton, T. A., and Rees, D. C. "OCAM: A New Tool for Studying the Oligomeric Diversity of MscL Channels" Protein Science 20, 313-326 (2011). doi:10.1002/pro.562 PUBMED

Liu, Z., Walton, T. A., and Rees, D. C. “A Reported Archaeal Mechanosensitive Channel is a Structural Homolog of MarR-1” Protein Science 19, 808-814 (2010). doi:10.1002/pro.360 PUBMED

Lewinson, O, Lee, A. T., Locher, K. P., and Rees, D. C. “A Distinct Mechanism for the ABC Transporter BtuCD-BtuF Revealed by the Dynamics of Complex Formation” Nature Struct. Molec. Biol. 17, 332-338 (2010). doi:10.1038/nsmb.1770 PUBMED

Liu, Z., Gandhi, C., and Rees, D. C. "Structure of a Tetrameric MscL in an Expanded Intermediate State" Nature 461, 120-126 (2009).
doi:10.1038/nature08277 PUBMED

Lewinson, O., Lee, A. T., and Rees, D. C. “A P-type ATPase Importer that Discriminates between Essential and Toxic Transition Metals” Proc. Natl. Acad. Sci. USA 106, 4677-4682 (2009). doi:10.1073/pnas.0900666106 PUBMED

Rees, D. C., Johnson, E., and Lewinson, O. "ABC Transporters: The Power to Change" Nature Reviews Molecular Cell Biology 10, 218-227 (2009).
doi:10.1038/nrm2646 PUBMED

Rice, A. E., Mendez, M. J., Hokanson, C. A., Rees, D. C., and Bjorkman, P. J. "Investigation of the Biophysical and Cell Biological Properties of Ferroportin, a Multipass Integral Membrane Protein Iron Exporter" J. Mol. Biol. 386, 717-732 (2009). doi:10.1016/j.jmb.2008.12.063

Gandhi, C. S. and Rees, D. C. "Opening the Molecular Floodgates" Science 321, 1166-116 (2008). doi:10.1126/science.1162963

Heijne, G. and Rees, D. "Membranes: Reading between the Lines" Curr. Op. Struct. Biol. 18, 403-405 (2008).

Kadaba, N. S., Kaiser, J. T., Johnson, E., Lee, A., and Rees, D. C. “The High-Affinity E. coli Methionine ABC Transporter: Structure and Allosteric Regulation” Science 321, 250-253 (2008). doi:10.1126/science.1157987 PUBMED

Lewinson, O., Lee, A. T., and Rees, D. C. “The Funnel Approach to the Precrystallization Production of Membrane Proteins” J. Mol. Biol. 377, 63-73 (2008). doi:10.1016/j.jmb.2007.12.059

Steinbacher, S., Bass, R., Strop, P., and Rees, D. C. “Structures of the Prokaryotic Mechanosensitive Channels MscL and MscS” Current Topics in Membranes in Mechanosensitive Ion Channels, Part A (Hamill, O. P. ed) volume 58, 1-24 (2007). doi:10.1016/S1063-5823(06)58001-9

Einsle, O., Andrade, S. L. A., Dobbek, H., Meyer, J., and Rees, D. C. “Assignment of Individual Metal Redox States in a Metalloprotein by Crystallographic Refinement at Multiple X-ray Wavelengths” J. Amer. Chem. Soc. 129, 2210-2211 (2007). doi:10.1021/ja067562o PUBMED

Pinkett, H. W., Lee, A. T., Lum, P., Locher, K. P., and Rees, D. C. “An Inward-Facing Conformation of a Metal-Chelate Type ABC Transporter” Science 315, 373-377 (2007). doi:10.1126/science.1133488

Howard, J. B. and Rees, D. C. “How Many Metals Does it Take to Fix N2? A Mechanistic Overview of Biological Nitrogen Fixation” Prof. Natl. Acad. Sci. USA 103, 17088-17093 (2006). doi:10.1073/PNAS.0603978103

Poolman, B., Doeven, M. K., Geertsma, E. R., Biemans-Oldenhinkel, E., Konings, W. N., and Rees, D. C. “Functional Analysis of Detergent-Solubilized and Membrane-Reconstituted ATP-Binding Cassette Transporters” Meth. Enzymol. 40,429-459 (2005). doi:10.1016/S0076-6879(05)00025-X

Borths, E. L., Poolman, B., Hvorup, R. N., Locher, K. P., and Rees, D. C. “In Vitro Functional Characterization of BtuCD-F, the Escherichia coli ABC Transporter for Vitamin B12 Uptake” Biochemistry 44, 16301-16309 (2005). doi:10.1021/bi0513103

Tezcan, F. A., Kaiser, J. T., Mustafi, D., Walton, M. Y., Howard, J. B., and Rees, D. C. “Nitrogenase Complexes: Multiple Docking Sites for a Nucleotide Switch Protein” Science 309, 1377-1380 (2005). doi:10.1126/science.1115653

Andrade, S. L. A., Cruz, F., Drennan, C. L., Ramakrishnan, V., Rees, D. C., Ferry, J. G., and Einsle, O. “Structures of the Iron-Sulfur Flavoproteins from Methanosarcina thermophila and Archaeoglobus fulgidus" J. Bacteriol. 187, 3848-3854 (2005). doi:10.1128/JB.187.11.3848-3854.2005

Rees, D. C., Tezcan, F. A., Haynes, C. A., Walton, M. Y., Andrade, S., Einsle, O., and Howard, J. B. “Structural Basis of Biological Nitrogen Fixation” Phil. Trans. R. Soc. A 363, 971-984 (2005). doi:10.1098/rsta.2004.1539

Corbett, M. C., Tezcan, F. A., Einsle, O., Walton, M. Y., Rees, D. C., Latimer, M. J., Hedman, B., and Hodgson, K. O. “Mo K– and L–edge X-ray Absorption Spectroscopic Study of the ADP•AIF4– -stabilized Nitrogenase Complex: Comparison with MoFe Protein in Solution and Single Crystal” J. Synchrotron Rad. 12, 28-34 (2005). doi:10.1107/S0909049504027827

Becker, C. F. W., Strop, P., Bass, R. B., Hansen, K. C., Locher, K. P., Ren, G., Yaeger, M., Rees, D. C., and Kochendoerfer, G. G. "Conversion of a Mechanosensitive Channel Protein from a Membrane-Embedded to a Water-Soluble form by Covalent Modification with Amphiphiles" J. Mol. Biol. 343, 747-758 (2004). doi:10.1016/j.jmb.2004.08.062

Nikolic-Hughes, I, Rees, D. C., and Herschlag, D. "Do Electrostatic Interactions with Positively Charged Active Site Groups Tighten the Transition State for Enzymatic Phosphoryl Transfer?" J. Am. Chem. Soc. 126, 11814-11819 (2004). doi:10.1021/ja0480421

Ambroggio, X. I., Rees, D. C., and Deshaies, R. "Jamm: A Metalloprotease-Like Zinc Site in the Proteasome and Signalosome" PLOS Biology 2, 113-119 (2004). doi:10.1371/journal.pbio.0020002

Bass, R. B., Locher, K. P., Borths, E., Poon, Y., Strop, P., Lee, A., and Rees, D. C. "The Structures of BtuCD and MscS and their Implications for Transporter and Channel Function" FEBS Lett. 555, 111-115 (2003). doi:10.1016/S0014-5793(03)01126-8

Perozo, E. and Rees, D. C. “Structure and Mechanism in Prokaryotic Mechanosensitive Channels” Curr. Op. Struct. Biol. 13, 432-442 (2003). doi:10.1016/S0959-440X(03)00106-4

Locher, K. P., Bass, R. B., and Rees, D. C. “Breaching the Barrier” Science 301, 603-604 (2003).

Rees, D. C. and Howard, J. B. “The Interface between the Biological and Inorganic Worlds: Iron-Sulfur Metalloclusters” Science 300, 929-931 (2003).

Shapovalov, G., Bass, R., Rees, D. C., and Lester, H. A. “Open-State Disulfide Crosslinking between Mycobacterium tuberculosis Mechanosensitive Channel Subunits” Biophys. J. 84, 2357-2365 (2003).

Strop, P., Bass, R., and Rees, D. C. “Prokaryotic Mechanosensitive Channels” in Advances in Protein Chemistry 63, 117-209 (2003).

Borths, E. L., Locher, K. P., Lee, A. T., and Rees, D. C. "The Structure of Escherichia coli BtuF and Binding to its Cognate ATP Binding Cassette Transporter" Proc. Natl. Acad. Sci. USA 99, 16642-16647 (2002). doi:10.1073/pnas.262659699

Bass, R. B., Strop, P., Barclay, M., and Rees, D. C. “Crystal Structure of Escherichia coli MscS, a Voltage-Modulated and Mechanosensitive Channel” Science 298, 1582-1587 (2002).

Schmid. B., Einsle, O., Chiu, H.-J. Willing, A., Yoshida, M., Howard, J. B., and Rees, D. C. "Biochemical and Structural Characterization of the Cross-linked Complex of Nitrogenase: Comparison to the ADP-AIF4- Stabilized Structure" Biochemistry 41, 15557-15565 (2002). doi:10.1021/bi026642b

Einsle, O., Tezcan, A., Andrade, S. L. A., Schmid, B., Yoshida, M., Howard, J. B., and Rees, D. C. “Nitrogenase MoFe-Protein at 1.16 Å Resolution: A Central Ligand in the FeMo-Cofactor” Science 297, 1696-1700 (2002).

Rees, D. C. "Great Metalloclusters in Enzymology" Ann. Rev. Biochem. 71, 221-246 (2002) doi:10.1146/annurev.biochem.71.110601.135406

Yeh, A. P., Ambroggio, X. I., Andrade, S. L. A., Einsle, O., Chatelet, C., Meyer, J., and Rees, D. C. “High Resolution Crystal Structures of the Wild Type and Cys55 -> Ser and Cys59 -> Ser Variants of the Thioredoxin-like [2Fe-2S] Ferredoxin from Aquifex aeolicus” J. Biol. Chem. 277, 3499-34507 (2002). doi:10.1074/jbc.M205096200

Axelrod, H. L., Abresch, E. C., Okamura, M. Y., Yeh, A. P., Rees, D. C., and Feher, G. (2002) “X-ray Structure Determination of the Cytochrome c2-Reaction Center Electron Transfer Complex from Rhodobacter sphaeroides” J. Mol. Biol. 319, 501-515 (2002).

Locher, K. P., Lee, A. T., and Rees, D. C. "The E. coli BtuCD Structure: A Framework for ABC Transporter Architecture and Mechanism" Science 296, 1091-1098 (2002).

Schmid, B., Ribbe, M. W., Einsle, O., Yoshida, M., Thomas, L. M., Dean, D. R., Rees, D. C., and Burgess, B. K. "Structure of a Cofactor-Deficient Nitrogenase MoFe Protein" Science 296, 352-356 (2002).

Spencer, R. H. and Rees, D. C. "The alpha-Helix and the Organization and Gating of Channels" Ann. Rev. Biophys. Biomol. Struct. 31, 207-233 (2002).

Rees, D. C. "Metalloproteins to Membrane Proteins: Biological Energy Transduction Mechanisms" in Structures and Mechanisms: From Ashes to Enzymes (Eaton, G. R., Wiley, D. C., and Jardetzky, O., eds.) ACS Symp. Series 827, Oxford University Press (2002).

Iverson, T. A., Luna-Chavez, C., Croal, L. R., Cecchini, G., and Rees, D. C. "Crystallographic Studies of the Escherichia coli Quinol-Fumerate Reductase with Inhibitors Bound to the Quinol-binding Site" J. Biol. Chem. 277, 16124-16130 (2002).